Characterization of the Involvement of Integrins, Focal Adhesion Kinase, and Phospholipase C Enzymes Endogenous to the Oocyte in Bovine Fertilization and Oocyte Activation
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چکیده
Characterization of the Involvement of Integrins, Focal Adhesion Kinase, and Phospholipase C Enzymes Endogenous to the Oocyte in Bovine Fertilization and Oocyte Activation
منابع مشابه
O-15: Reduced Fertilization After ICSI and Abnormal Phospholipase C Zeta Presence in Spermatozoa from the Wobbler Mouse
Background: Failed fertilization after intracytoplasmic sperm injection (ICSI) can be due to a reduced oocyte-activation capacity caused by reduced concentrations and abnormal localization of the oocyte-activation factor phospholipase C (PLC) zeta. Patients with this condition can be helped to conceive by artificial activation of oocytes after ICSI with calcium ionophore (assisted oocyte activa...
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Background: Failed fertilization post ICSI has been mainly attributed to the sperm’s inability to induce oocyte activation. Phospholipase C Zeta (PLCζ) is considered to be a primary factor for the induction of oocyte activation. The aim of this study was to quantitatively assess the expression of PLCζ in globozoospermic or individuals with previous low or failed fertilization in comparison to f...
متن کاملI-15: Assessment of Transcript and Protein Profiles of Infertile Individual May Help to Select Individuals with Low Fertilization Potential Candidate of Artificial Oocyte Activation
Background Following sperm penetration, oocyte is activated by sperm oocyte activating factors (SOAFs) released by sperm. Spermspecific phospholipase C isoform ζ (PLCζ) and post acrosomal WW binding protein (PAWP) are two nominees for the SOAF. PLCζ is located back-to-back with another testis-specific gene called CAPZA3. These two genes share a common bidirectional promoter. In this study we as...
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Low and/or total failed fertilization following ICSI is a disappointing effect that some couples may face during their treatment which may have severe social and economic consequences for these infertile couples. This phenomenon has been mainly attributed to failed oocyte activation post ICSI. Researchers have regarded this failure due to the absence or deficiency of sperm associated to oocyte ...
متن کاملI-8: Role of Sperm Transcripts in Oocyte Activation, Fertilization and Development
It is well established that in addition to generating the diploid state in the zygote, the sperm also endows the oocyte with paternal centrioles, proteins and RNAs. Although the exact roles of mRNA introduced into the oocyte by the sperm are not completely understood. However, literature background, suggested that RNAs may have a vital role in embryo cleavage and further development. Therefore,...
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